[gmx-users] desired no of water molecules

Peter C. Lai pcl at uab.edu
Tue Aug 14 07:05:58 CEST 2012


hmm. What happens if you try -maxsol instead of -nmol? 

On 2012-08-14 10:24:18AM +0530, tarak karmakar wrote:
> This is the ala.gro (alanine dipeptide)  file which does not contain
> any water (SOL) a priori.
> 
> Grunge ROck MAChoS
>    22
>     1ACE   HH31    1   1.267   1.058   1.673
>     1ACE    CH3    2   1.364   1.086   1.631
>     1ACE   HH32    3   1.422   0.996   1.607
>     1ACE   HH33    4   1.416   1.148   1.705
>     1ACE      C    5   1.348   1.183   1.515
>     1ACE      O    6   1.381   1.152   1.401
>     2ALA      N    7   1.301   1.303   1.549
>     2ALA      H    8   1.284   1.324   1.646
>     2ALA     CA    9   1.290   1.409   1.450
>     2ALA     HA   10   1.374   1.399   1.381
>     2ALA     CB   11   1.160   1.402   1.371
>     2ALA    HB1   12   1.131   1.299   1.351
>     2ALA    HB2   13   1.086   1.455   1.431
>     2ALA    HB3   14   1.166   1.447   1.272
>     2ALA      C   15   1.301   1.548   1.510
>     2ALA      O   16   1.277   1.567   1.629
>     3NME      N   17   1.337   1.646   1.426
>     3NME      H   18   1.356   1.619   1.330
>     3NME    CH3   19   1.344   1.784   1.469
>     3NME   HH31   20   1.408   1.835   1.398
>     3NME   HH32   21   1.250   1.838   1.469
>     3NME   HH33   22   1.394   1.797   1.565
>    0.33600   0.84200   0.43300
> 
> 
> If I grep 'SOL' ala_solv.gro , then it is showing
> 
>     4SOL     OW   23   0.569   1.275   1.165
>     4SOL    HW1   24   0.476   1.268   1.128
>     4SOL    HW2   25   0.580   1.364   1.209
> ......................................
> ...................................
>   668SOL     OW 2015   0.755   0.751   0.211
>   668SOL    HW1 2016   0.785   0.679   0.149
>   668SOL    HW2 2017   0.716   0.711   0.294
>   669SOL     OW 2018   2.632   0.122   2.163
>   669SOL    HW1 2019   2.586   0.035   2.180
>   669SOL    HW2 2020   2.723   0.119   2.204
>   670SOL     OW 2021   0.144   1.914   2.030
>   670SOL    HW1 2022   0.179   1.873   1.946
>   670SOL    HW2 2023   0.054   1.876   2.050
>   671SOL     OW 2024   1.901   2.939   2.162
>   671SOL    HW1 2025   2.000   2.928   2.153
>   671SOL    HW2 2026   1.861   2.853   2.194
> 
> so certainly it does contain 668 water molecules.
> Thanks,
> 
> On Tue, Aug 14, 2012 at 10:13 AM, Peter C. Lai <pcl at uab.edu> wrote:
> > Did you have waters in the system before adding another 612?
> >
> > I don't know how VMD counts waters, but another way is to grep -c for SOL in
> > the resulting gro file and divide by 3.
> >
> > On 2012-08-14 10:07:54AM +0530, tarak karmakar wrote:
> >> Thanks for the quick reply, I have given this command to add 612 water
> >> molecules.
> >> genbox -cs spc216 -nmol 612 -cp ala_box.gro -o ala_solv.gro -p ala.top
> >>
> >> But in the solvated file, I see there are 668 water molecules.
> >> A part of the output while adding water molecules is as follows
> >>
> >>  Using plugin gro for structure file ala_solv.gro
> >> Info) Using plugin gro for coordinates from file ala_solv.gro
> >> Info) Determining bond structure from distance search ...
> >> Info) Finished with coordinate file ala_solv.gro.
> >> Info) Analyzing structure ...
> >> Info)    Atoms: 2026
> >> Info)    Bonds: 1357
> >> Info)    Angles: 0  Dihedrals: 0  Impropers: 0  Cross-terms: 0
> >> Info)    Bondtypes: 0  Angletypes: 0  Dihedraltypes: 0  Impropertypes: 0
> >> Info)    Residues: 671
> >> Info)    Waters: 668
> >> Warning) Unusual bond between residues:  1 (none) and 2 (protein)
> >> Warning) Unusual bond between residues:  2 (protein) and 3 (none)
> >> Info)    Segments: 1
> >> Info)    Fragments: 669   Protein: 1   Nucleic: 0
> >>
> >>
> >> On Tue, Aug 14, 2012 at 9:46 AM, Mark Abraham <Mark.Abraham at anu.edu.au> wrote:
> >> > On 14/08/2012 2:02 PM, tarak karmakar wrote:
> >> >>
> >> >> Dear All,
> >> >>
> >> >> Is there any way to add a specific number of water ( let say 650
> >> >> water) molecules while dissolving the solute in a given box ?
> >> >
> >> >
> >> > Check out genbox -h
> >> >
> >> > Mark
> >> > --
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> >>
> >>
> >> Tarak
> >> --
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> > --
> > ==================================================================
> > Peter C. Lai                    | University of Alabama-Birmingham
> > Programmer/Analyst              | KAUL 752A
> > Genetics, Div. of Research      | 705 South 20th Street
> > pcl at uab.edu                     | Birmingham AL 35294-4461
> > (205) 690-0808                  |
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> 
> Tarak
> -- 
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-- 
==================================================================
Peter C. Lai			| University of Alabama-Birmingham
Programmer/Analyst		| KAUL 752A
Genetics, Div. of Research	| 705 South 20th Street
pcl at uab.edu			| Birmingham AL 35294-4461
(205) 690-0808			|
==================================================================




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