[gmx-users] Protein-POPC bilayer

[Shima Arasteh]

 Hi,

I have a question about the Protein-POPC system:
To insert a protein in lipid bilayer, I am suggested to simulate POPC in water separately before insertion, it might decrease the time of final simulation. It's OK!

In the article suggested me by dear Peter C. Lai, I read that POPC was simulated in anisotropic pressure coupling at first and then after insertion of protein, semi-isotropic pressure coupling is applied. 
Now, would you please telling me why you used this procedure?
And,
Would my system be correct  if I use semi-isotropic pressure coupling instead of anisotropic pressure coupling for the first step?

Thanks in advance for your replies.


Sincerely,
Shima