[gmx-users] Atom O5' not found in residue seq.nr. 1 while adding atom

mahr mahr at bio.gnu.ac.kr
Fri May 18 07:09:04 CEST 2012


 Dear Gromacs Users,

                        I m trying to simulate a DNA structure with amber03
force field. However, when I give first command of pdb2gmx.

Following error comes:

Fatal error: Atom O5' not found in residue seq.nr. 1 while adding atom.

As O5' is present in DA nucleic acid. So,I checked DNA.rtp file and
nomenclature of DA, DT and all other nucleic acid residues is same for both
.pdb and .rtp file.

Plz plz guide me how to solve this problem.

Following is my .rtp file for DA

[ DA ]

 [ atoms ]

     P    P             1.16590     1

   O1P    O2           -0.77610     2

   O2P    O2           -0.77610     3

   O5'    OS           -0.49540     4

   C5'    CT           -0.00690     5

  H5'1    H1            0.07540     6

  H5'2    H1            0.07540     7

   C4'    CT            0.16290     8

   H4'    H1            0.11760     9

   O4'    OS           -0.36910    10

   C1'    CT            0.04310    11

   H1'    H2            0.18380    12

    N9    N*           -0.02680    13

    C8    CK            0.16070    14

    H8    H5            0.18770    15

    N7    NB           -0.61750    16

    C5    CB            0.07250    17

    C6    CA            0.68970    18

    N6    N2           -0.91230    19

   H61    H             0.41670    20

   H62    H             0.41670    21

    N1    NC           -0.76240    22

    C2    CQ            0.57160    23

    H2    H5            0.05980    24

    N3    NC           -0.74170    25

    C4    CB            0.38000    26

   C3'    CT            0.07130    27

   H3'    H1            0.09850    28

   C2'    CT           -0.08540    29

  H2'1    HC            0.07180    30

  H2'2    HC            0.07180    31

   O3'    OS           -0.52320    32

 [ bonds ]

     P   O1P

     P   O2P

     P   O5'

   O5'   C5'

   C5'  H5'1

   C5'  H5'2

   C5'   C4'

   C4'   H4'

   C4'   O4'

   C4'   C3'

   O4'   C1'

   C1'   H1'

   C1'    N9

   C1'   C2'

    N9    C8

    N9    C4

    C8    H8

    C8    N7

    N7    C5

    C5    C6

    C5    C4

    C6    N6

    C6    N1

    N6   H61

    N6   H62

    N1    C2

C2    H2

    C2    N3

    N3    C4

    C3'   H3'

    C3'   C2'

    C3'   O3'

    C2'  H2'1

    C2'  H2'2

    -O3'     P

 [ impropers ]

    C4    C8    N9   C1'

    C6   H61    N6   H62

    N9    N7    C8    H8

    N1    N3    C2    H2

    C5    N6    C6    N1

 

Following is DA part of my pdb file:

 

ATOM     28    P   DA E   10    -24.026  32.156 -43.818  1.00180.08
P

ATOM     29  O1P   DA E   10    -23.715  31.139 -42.788  1.00173.83
O

ATOM     30  O2P   DA E   10    -25.435  32.499 -44.114  1.00161.01
O

ATOM     31  O5'   DA E   10    -23.255  33.516 -43.475  1.00127.88     O

ATOM     32  C5'   DA E   10    -23.498  34.194 -42.247  1.00107.59
C

ATOM     33  C4'   DA E   10    -23.169  33.299 -41.066  1.00102.86
C

ATOM     34  O4'   DA E   10    -21.825  32.777 -41.221  1.00 93.42
O

ATOM     35  C3'   DA E   10    -23.220  33.983 -39.705  1.00102.18
C

ATOM     36  O3'   DA E   10    -23.829  33.119 -38.754  1.00 83.31
O

ATOM     37  C2'   DA E   10    -21.755  34.244 -39.363  1.00102.50
C

ATOM     38  C1'   DA E   10    -21.012  33.153 -40.128  1.00 81.75
C

ATOM     39  N9    DA E   10    -19.721  33.596 -40.647  1.00 82.20
N

ATOM     40  C8    DA E   10    -19.495  34.603 -41.544  1.00 94.12
C

ATOM     41  N7    DA E   10    -18.227  34.783 -41.832  1.00102.29
N

ATOM     42  C5    DA E   10    -17.576  33.827 -41.070  1.00 96.10
C

ATOM     43  C6    DA E   10    -16.215  33.496 -40.924  1.00 87.15
C

ATOM     44  N6    DA E   10    -15.230  34.125 -41.572  1.00 84.12
N

ATOM     45  N1    DA E   10    -15.903  32.488 -40.082  1.00 75.71
N

ATOM     46  C2    DA E   10    -16.891  31.860 -39.434  1.00 80.51
C

ATOM     47  N3    DA E   10    -18.203  32.081 -39.490  1.00 91.21
N

ATOM     48  C4    DA E   10    -18.482  33.087 -40.334  1.00 96.39
C

ATOM     49 H5'1   DA E   10    -22.876  35.088 -42.203  1.00105.06
H

ATOM     50 H5'2   DA E   10    -24.549  34.479 -42.198  1.00105.06
H

ATOM     51  H4'   DA E   10    -23.867  32.462 -41.060  1.00105.06
H

ATOM     52  H3'   DA E   10    -23.768  34.923 -39.772  1.00105.06
H

ATOM     53 H2'1   DA E   10    -21.447  35.234 -39.699  1.00105.06
H

ATOM     54 H2'2   DA E   10    -21.583  34.154 -38.290  1.00105.06
H

ATOM     55  H1'   DA E   10    -20.863  32.292 -39.476  1.00105.06
H

ATOM     56  H8    DA E   10    -20.295  35.202 -41.980  1.00105.06
H

ATOM     57  H61   DA E   10    -15.458  34.926 -42.244  1.00105.06
H

ATOM     58  H62   DA E   10    -14.214  33.827 -41.419  1.00105.06
H

ATOM     59  H2    DA E   10    -16.579  31.056 -38.768  1.00105.06
H

 

I will be very thankful. 

 

Best regards

mahreen

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