Hello, At NPT stage the two leaflets in DMPC bilayer is separated a while and comes closer. Is this common in this stage or any thing goes wrong in equillibration. Thanks --Rama -- View this message in context: http://gromacs.5086.x6.nabble.com/DMPC-Bilayer-tp5010783.html Sent from the GROMACS Users Forum mailing list archive at Nabble.com.