[gmx-users] Error in system_inflate.gro coordinates does not match

RINU KHATTRI nickname.mittu at gmail.com
Wed Aug 6 12:12:25 CEST 2014


hello every one
i am working on complex with popc membrane i got error in Deuterium
Order Parameters group 1 does not have same number of elements as grp
1
i used the command

make_ndx -f md_0_1.tpr -o sn1.ndx
0 System              : 38483 atoms
  1 Protein             :  3873 atoms
  2 Protein-H           :  3061 atoms
  3 C-alpha             :   408 atoms
  4 Backbone            :  1224 atoms
  5 MainChain           :  1633 atoms
  6 MainChain+Cb        :  2015 atoms
  7 MainChain+H         :  2000 atoms
  8 SideChain           :  1873 atoms
  9 SideChain-H         :  1428 atoms
 10 Prot-Masses         :  3873 atoms
 11 non-Protein         : 34610 atoms
 12 Other               :  6365 atoms
 13 UNK                 :    21 atoms
 14 POPC                :  6344 atoms
 15 CL                  :    15 atoms
 16 Water               : 28230 atoms
 17 SOL                 : 28230 atoms
 18 non-Water           : 10253 atoms
 19 Ion                 :    15 atoms
 20 UNK                 :    21 atoms
 21 POPC                :  6344 atoms
 22 CL                  :    15 atoms
 23 Water_and_ions      : 28245 atoms

 nr : group       !   'name' nr name   'splitch' nr    Enter: list groups
 'a': atom        &   'del' nr         'splitres' nr   'l': list residues
 't': atom type   |   'keep' nr        'splitat' nr    'h': help
 'r': residue         'res' nr         'chain' char
 "name": group        'case': case sensitive           'q': save and quit
 'ri': residue index

> aC34

Found 122 atoms with name C34

 24 C34                 :   122 atoms

> aC36

Found 122 atoms with name C36

 25 C36                 :   122 atoms

> a C37

Found 122 atoms with name C37

 26 C37                 :   122 atoms

> a C38

Found 122 atoms with name C38

 27 C38                 :   122 atoms

> a C39

Found 122 atoms with name C39

 28 C39                 :   122 atoms

> a C40

Found 122 atoms with name C40

 29 C40                 :   122 atoms

> a C41

Found 122 atoms with name C41

 30 C41                 :   122 atoms

> a C42

Found 122 atoms with name C42

 31 C42                 :   122 atoms

> A C43


Syntax error: "A C43"

> a C43

Found 122 atoms with name C43

 32 C43                 :   122 atoms

> a C44

Found 122 atoms with name C44

 33 C44                 :   122 atoms

> a C45

Found 122 atoms with name C45

 34 C45                 :   122 atoms

> a C46

Found 122 atoms with name C46

 35 C46                 :   122 atoms

> a C47

Found 122 atoms with name C47

 36 C47                 :   122 atoms

> a C48

Found 122 atoms with name C48

 37 C48                 :   122 atoms

> a C49

Found 122 atoms with name C49

 38 C49                 :   122 atoms

> a C50

Found 122 atoms with name C50

 39 C50                 :   122 atoms

> a CA1

Found 122 atoms with name CA1

 40 CA1                 :   122 atoms

> a CA2

Found 122 atoms with name CA2

 41 CA2                 :   122 atoms

> del 0-21

Removed group 0 'System'
Removed group 1 'Protein'
Removed group 2 'Protein-H'
Removed group 3 'C-alpha'
Removed group 4 'Backbone'
Removed group 5 'MainChain'
Removed group 6 'MainChain+Cb'
Removed group 7 'MainChain+H'
Removed group 8 'SideChain'
Removed group 9 'SideChain-H'
Removed group 10 'Prot-Masses'
Removed group 11 'non-Protein'
Removed group 12 'Other'
Removed group 13 'UNK'
Removed group 14 'POPC'
Removed group 15 'CL'
Removed group 16 'Water'
Removed group 17 'SOL'
Removed group 18 'non-Water'
Removed group 19 'Ion'
Removed group 20 'UNK'
Removed group 21 'POPC'
> q
 0 System              : 38483 atoms
  1 Protein             :  3873 atoms
  2 Protein-H           :  3061 atoms
  3 C-alpha             :   408 atoms
  4 Backbone            :  1224 atoms
  5 MainChain           :  1633 atoms
  6 MainChain+Cb        :  2015 atoms
  7 MainChain+H         :  2000 atoms
  8 SideChain           :  1873 atoms
  9 SideChain-H         :  1428 atoms
 10 Prot-Masses         :  3873 atoms
 11 non-Protein         : 34610 atoms
 12 Other               :  6365 atoms
 13 UNK                 :    21 atoms
 14 POPC                :  6344 atoms
 15 CL                  :    15 atoms
 16 Water               : 28230 atoms
 17 SOL                 : 28230 atoms
 18 non-Water           : 10253 atoms
 19 Ion                 :    15 atoms
 20 UNK                 :    21 atoms
 21 POPC                :  6344 atoms
 22 CL                  :    15 atoms
 23 Water_and_ions      : 28245 atoms

 nr : group       !   'name' nr name   'splitch' nr    Enter: list groups
 'a': atom        &   'del' nr         'splitres' nr   'l': list residues
 't': atom type   |   'keep' nr        'splitat' nr    'h': help
 'r': residue         'res' nr         'chain' char
 "name": group        'case': case sensitive           'q': save and quit
 'ri': residue index

> a C15

Found 122 atoms with name C15

 24 C15                 :   122 atoms

> a C17

Found 122 atoms with name C17

 25 C17                 :   122 atoms

> a C18

Found 122 atoms with name C18

 26 C18                 :   122 atoms

> a C19

Found 122 atoms with name C19

 27 C19                 :   122 atoms

> a C20

Found 122 atoms with name C20

 28 C20                 :   122 atoms

> a C21

Found 122 atoms with name C21

 29 C21                 :   122 atoms

> a C22

Found 122 atoms with name C22

 30 C22                 :   122 atoms

> a C23

Found 122 atoms with name C23

 31 C23                 :   122 atoms

> a C24

Found 122 atoms with name C24

 32 C24                 :   122 atoms

> a C25

Found 122 atoms with name C25

 33 C25                 :   122 atoms

> a C26

Found 122 atoms with name C26

 34 C26                 :   122 atoms

> a C27

Found 122 atoms with name C27

 35 C27                 :   122 atoms

> a C28

Found 122 atoms with name C28

 36 C28                 :   122 atoms

> a C29

Found 122 atoms with name C29

 37 C29                 :   122 atoms

> a C30

Found 122 atoms with name C30

 38 C30                 :   122 atoms

> a C31

Found 122 atoms with name C31

 39 C31                 :   122 atoms

> q

mtechbio at mtechbio-HP-Z400-Workstation:~/Desktop/pt1/pt1/epi$ g_order_d
-s md_0_1.tpr -f md_0_1.xtc -n sn1.ndx -d z -od deuter_sn1.xvg


Taking z axis as normal to the membrane
Reading file md_0_1.tpr, VERSION 4.5.5 (single precision)
Using following groups:
Groupname: CL First atomname: CL First atomnr 38468
Groupname: Water_and_ions First atomname: OW First atomnr 10238
Groupname: C34 First atomname: C34 First atomnr 3927
Groupname: C36 First atomname: C36 First atomnr 3929
Groupname: C37 First atomname: C37 First atomnr 3930
Groupname: C38 First atomname: C38 First atomnr 3931
Groupname: C39 First atomname: C39 First atomnr 3932
Groupname: C40 First atomname: C40 First atomnr 3933
Groupname: C41 First atomname: C41 First atomnr 3934
Groupname: C42 First atomname: C42 First atomnr 3935
Groupname: C43 First atomname: C43 First atomnr 3936
Groupname: C44 First atomname: C44 First atomnr 3937
Groupname: C45 First atomname: C45 First atomnr 3938
Groupname: C46 First atomname: C46 First atomnr 3939
Groupname: C47 First atomname: C47 First atomnr 3940
Groupname: C48 First atomname: C48 First atomnr 3941
Groupname: C49 First atomname: C49 First atomnr 3942
Groupname: C50 First atomname: C50 First atomnr 3943
Groupname: CA1 First atomname: CA1 First atomnr 3944
Groupname: CA2 First atomname: CA2 First atomnr 3945

Reading frame       0 time    0.000   Number of elements in first group: 15

-------------------------------------------------------
Program g_order_d, VERSION 4.5.5
Source code file: /build/buildd/gromacs-4.5.5/src/tools/gmx_order.c, line: 489

Fatal error:
grp 1 does not have same number of elements as grp 1
kindly help
i didnt get the Dallas  Warner post

On Mon, Jul 28, 2014 at 5:24 PM, Justin Lemkul <jalemkul at vt.edu> wrote:
>
>
> On 7/28/14, 1:42 AM, RINU KHATTRI wrote:
>>
>> hello everyone
>>
>> i used editconf
>>
>> editconf -f protein.gro -o protein_newbox.gro -box (membrane box
>> vectors) -center *x y z*
>>
>>
>> but in z axis i have been increased box size 10.0000 (previously it is
>> 6.23910   6.17970   6.91950
>>
>> http://s48.photobucket.com/user/mittukhattri/media/ri_zpsbb51fbd7.png.html?filters[user]=140927090&filters[recent]=1&sort=1&o=0
>>
>> this is the em.gro first minimization image
>>
>>   mixing of lipid and protein molecule
>>
>> kindly help
>>
>
> I don't see any evidence of a problem.
>
>
> -Justin
>
> --
> ==================================================
>
> Justin A. Lemkul, Ph.D.
> Ruth L. Kirschstein NRSA Postdoctoral Fellow
>
> Department of Pharmaceutical Sciences
> School of Pharmacy
> Health Sciences Facility II, Room 601
> University of Maryland, Baltimore
> 20 Penn St.
> Baltimore, MD 21201
>
> jalemkul at outerbanks.umaryland.edu | (410) 706-7441
> http://mackerell.umaryland.edu/~jalemkul
>
> ==================================================
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