Hello , can anyone tell me why im getting profile like this after umbrella simulation im attaching both hist.xvg and profile.xvg plot histo plot https://drive.google.com/file/d/0BxaQk_pcR9viaGRNQlhrcVVmazQ/view?usp=sharing profile https://drive.google.com/file/d/0BxaQk_pcR9viMHNOTE5Ba3VlY2c/view?usp=sharing Thanks Nikhil