[gmx-users] Simulate protein at subzero condition in aqueous buffer

[ZHANG Cheng]

Dear Gromacs,
I would like to simulate the protein at subzero condition in aqueous buffer, to see if it becomes more stable than the elevated temperature (e.g. 65 C). Can I ask what is the valid temperature range for water "spc216.gro" ? If I run the simulation at -40 C, does it still assume the system as liquid state instead of frozen state? Thank you.


Yours sincerely
Cheng