[gmx-users] gromacs.org_gmx-users Digest, Vol 168, Issue 45

Justin Lemkul jalemkul at vt.edu
Thu Apr 12 19:12:54 CEST 2018



On 4/12/18 2:55 AM, zaved at tezu.ernet.in wrote:
> Dear Mark
>
> Thank you for the kind response.
>
> H with +0.418 charge and N with -0.845 charge is contributing towards
> coulomb 14 interaction. So what is the amount of interaction between one
> single H and N (3 bonds apart)? Kindly correct me if I am wrong.

Simply calculate it with Coulomb's Law and apply the 1-4 electrostatic 
scaling factor (if your force field uses one).

-Justin

> Here is my Gdm topology
>
> [ moleculetype ]
> ; name  nrexcl
> GDM         3
>
> [ atoms ]
> ;   nr    type   resnr  residu    atom    cgnr  charge	MASS
>       1      C        1    GND       C1       1	 1.027	13.019
>       2     NT        1    GND       N2       2	 -0.845	14.0067
>       3      H        1    GND       H3       2	 0.418	1.008
>       4      H        1    GND       H4       2	 0.418	1.008
>       5     NT        1    GND       N5       3	 -0.845	14.0067
>       6      H        1    GND       H6       3   0.418	1.008
>       7      H        1    GND       H7       3	 0.418	1.008
>       8     NT        1    GND       N8       4	 -0.845	14.0067
>       9      H        1    GND       H9       4	 0.418	1.008
>       10     H        1    GND       H10      4	 0.418	1.008
>
>
>   [ bonds ]
>      1     2  2  0.1340  1.0500e+07
>      1     5  2  0.1340  1.0500e+07
>      1     8  2  0.1340  1.0500e+07
>      2     3  2  0.1000  1.8700e+07
>      2     4  2  0.1000  1.8700e+07
>      5     6  2  0.1000  1.8700e+07
>      5     7  2  0.1000  1.8700e+07
>      8     9  2  0.1000  1.8700e+07
>      8    10  2  0.1000  1.8700e+07
>
>
>   [ angles ]
>      1     2     3    2  120.00      390.00
>      1     2     4    2  120.00      390.00
>      1     5     6    2  120.00      390.00
>      1     5     7    2  120.00      390.00
>      1     8     9    2  120.00      390.00
>      1     8    10    2  120.00      390.00
>      3     2     4    2  120.00      445.00
>      6     5     7    2  120.00      445.00
>      9     8    10    2  120.00      445.00
>      2     1     5    2  120.00      670.00
>      5     1     8    2  120.00      670.00
>      8     1     2    2  120.00      670.00
>
>
> [ pairs ]
> ;  ai    aj funct           c0           c1
>      2     6     1 0.000000e+00 0.000000e+00
>      2     7     1 0.000000e+00 0.000000e+00
>      2     9     1 0.000000e+00 0.000000e+00
>      2    10     1 0.000000e+00 0.000000e+00
>      5     3     1 0.000000e+00 0.000000e+00
>      5     4     1 0.000000e+00 0.000000e+00
>      5     9     1 0.000000e+00 0.000000e+00
>      5    10     1 0.000000e+00 0.000000e+00
>      8     3     1 0.000000e+00 0.000000e+00
>      8     4     1 0.000000e+00 0.000000e+00
>      8     6     1 0.000000e+00 0.000000e+00
>      8     7     1 0.000000e+00 0.000000e+00
>
>   [ dihedrals ]
> ;  ai    aj    ak    al   gromos type
>      2     1     5     6  1 180.000       33.5          2
>      2     1     5     7  1 180.000       33.5          2
>      2     1     8     9  1 180.000       33.5          2
>      2     1     8    10  1 180.000       33.5          2
>      5     1     2     3  1 180.000       33.5          2
>      5     1     2     4  1 180.000       33.5          2
>      5     1     8     9  1 180.000       33.5          2
>      5     1     8    10  1 180.000       33.5          2
>      8     1     2     3  1 180.000       33.5          2
>      8     1     2     4  1 180.000       33.5          2
>
> Thank you
>
> Regards
> Zaved Hazarika
> PhD Scholar
> Dept.of Molecular Biology and Biotechnology
> Tezpur University
> India
>
>> Hi,
>>
>> Presumably Gdm has atoms connected by three adjacent bonds that generally
>> have negative potential, and there's enough Gdm to make the total for the
>> system negative. It's generally a good idea to understand how the topology
>> you're using for Gdm works.
>>
>> Mark
>>
>> On Tue, Apr 10, 2018 at 2:23 PM <zaved at tezu.ernet.in> wrote:
>>
>>> Dear Gromacs Users
>>>
>>> I have simulated ricin in presence of GdmCl (1519 Gdm and 1519 Cl) with
>>> gromos54a7 ff. Simulations have been performed for 100ns each at 298K,
>>> 350K and 450K.
>>>
>>> The Average Coulomb-14 Energies are as follows:
>>>
>>> ricin in water (298K) : 3.42E+04 (positive value)
>>> ricin in water (350K) : 3.41E+04 (positive value)
>>> ricin in water (450K) : 3.40E+04 (positive value)
>>>
>>> ricin + GdmCl  (298K)  :-3.16E+06 (negative value)
>>> ricin + GdmCl  (350K) : -3.17E+06 (negative value)
>>> ricin + GdmCl  (450K) : -3.17E+06 (negative value)
>>>
>>> Can anyone suggest me why there is a (10 to the power 10) fold
>>> difference
>>> of average Coulomb-14 energy of ricin in presence of water and ricin in
>>> presence of GdmCl?
>>>
>>> Thank You
>>>
>>> Regards
>>>
>>> Zaved
>
>
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-- 
==================================================

Justin A. Lemkul, Ph.D.
Assistant Professor
Virginia Tech Department of Biochemistry

303 Engel Hall
340 West Campus Dr.
Blacksburg, VA 24061

jalemkul at vt.edu | (540) 231-3129
http://www.thelemkullab.com

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