[gmx-users] bad bond in polymer

nicegromacs nicegromacs at live.cl
Thu Aug 13 05:57:13 CEST 2009


Well, I tried to build a polymer with 3 monomers, DRG DR0 and DR1. In the 
pdb file They have the same order (DRG DR0 DR1).  But when tried with 
pdb2gmx then I have the error:
Fatal error:
In the .rtp file in residue DR1 at line:
   CF1   CH3   0   1
I changed the order of monomers to DR0 DRG DR1 with respect to the ''head'' 
monomer (DR0), Monomer of in the middle (DRG), and tail monomer (DR1), and 
the error no change. I have not changed the .hdb file.

the estructure.ent
HETATM    1  CAA DRG     1      58.110  44.852  36.809
HETATM    2  CAB DRG     1      56.934  44.517  35.875
HETATM    3  CAC DRG     1      56.190  45.811  35.500
HETATM    4  CAD DRG     1      55.051  45.476  34.520
HETATM    5  CAE DRG     1      54.318  46.770  34.118
HETATM    6  CAF DRG     1      53.310  46.438  33.003
HETATM    7  CAG DRG     1      52.657  47.686  32.473
HETATM    8  CAH DRG     1      53.521  48.753  31.851
HETATM    9  CAI DRG     1      54.199  48.265  30.599
HETATM   10  CAJ DRG     1      55.615  47.887  30.630
HETATM   11  OAK DRG     1      56.231  47.370  29.495
HETATM   12  OAL DRG     1      56.359  48.028  31.798
HETATM   13  CAM DRG     1      53.423  48.169  29.314
HETATM   14  CAN DRG     1      52.529  46.963  29.306
HETATM   15  OAO DRG     1      53.058  45.698  29.542
HETATM   16  OAP DRG     1      51.206  47.090  28.902
HETATM   17  CA1 DR0     1      43.743  50.975  25.947
HETATM   18  CA2 DR0     1      44.674  51.739  26.906
HETATM   19  CA3 DR0     1      45.313  50.749  27.897
HETATM   20  CA4 DR0     1      46.248  51.508  28.856
HETATM   21  CA5 DR0     1      46.862  50.512  29.856
HETATM   22  CA6 DR0     1      47.789  51.264  30.830
HETATM   23  CA7 DR0     1      48.356  50.315  31.851
HETATM   24  CA8 DR0     1      49.361  49.293  31.385
HETATM   25  CA9 DR0     1      50.545  49.110  32.302
HETATM   26  C10 DR0     1      51.122  50.259  33.020
HETATM   27  O12 DR0     1      50.919  51.560  32.571
HETATM   28  O11 DR0     1      51.877  50.069  34.174
HETATM   29  C13 DR0     1      51.189  47.782  32.378
HETATM   30  C14 DR0     1      50.382  46.559  32.210
HETATM   31  O16 DR0     1      50.972  45.336  31.910
HETATM   32  O15 DR0     1      48.997  46.595  32.315
HETATM   33  CF1 DR1     1      55.642  46.172  39.128
HETATM   34  CF2 DR1     1      54.229  46.780  39.194
HETATM   35  CF3 DR1     1      53.591  46.765  37.791
HETATM   36  CF4 DR1     1      52.185  47.384  37.876
HETATM   37  CF5 DR1     1      51.526  47.395  36.484
HETATM   38  CF6 DR1     1      50.134  48.044  36.601
HETATM   39  CF7 DR1     1      49.475  48.098  35.212
HETATM   40  CF8 DR1     1      48.132  48.846  35.298
HETATM   41  CF9 DR1     1      47.562  49.035  33.918
HETATM   42  C1F DR1     1      46.647  48.028  33.366
HETATM   43  O3F DR1     1      46.397  46.849  34.062
HETATM   44  O2F DR1     1      45.998  48.241  32.154
HETATM   45  C4F DR1     1      47.798  50.304  33.215
HETATM   46  C5F DR1     1      47.426  51.565  33.874
HETATM   47  O7F DR1     1      47.843  52.791  33.365
HETATM   48  O6F DR1     1      46.661  51.551  35.036
CONECT    1    2
CONECT    2    1    3
CONECT    3    2    4
CONECT    4    3    5
CONECT    5    4    6
CONECT    6    5    7
CONECT    7    6    8   29
CONECT    8    7    9
CONECT    9    8   10   13
CONECT   10    9   11   12
CONECT   11   10
CONECT   12   10
CONECT   13    9   14
CONECT   14   13   15   16
CONECT   15   14
CONECT   16   14
CONECT   17   18
CONECT   18   17   19
CONECT   19   18   20
CONECT   20   19   21
CONECT   21   20   22
CONECT   22   21   23
CONECT   23   22   24   45
CONECT   24   23   25
CONECT   25   24   26   29
CONECT   26   25   27   28
CONECT   27   26
CONECT   28   26
CONECT   29   25   30    7
CONECT   30   29   31   32
CONECT   31   30
CONECT   32   30
CONECT   33   34
CONECT   34   33   35
CONECT   35   34   36
CONECT   36   35   37
CONECT   37   36   38
CONECT   38   37   39
CONECT   39   38   40
CONECT   40   39   41
CONECT   41   40   42   45
CONECT   42   41   43   44
CONECT   43   42
CONECT   44   42
CONECT   45   41   46   23
CONECT   46   45   47   48
CONECT   47   46
CONECT   48   46
END
 and the .rtp

[ DRG ]   ; unidad
 [ atoms ]
   CAA  CH3    0.00
   CAB  CH2    0.00
   CAC  CH2    0.00
   CAD  CH2    0.00
   CAE  CH2    0.00
   CAF  CH2    0.00
   CAG  CH1    0.00
   CAH  CH2    0.04
   CAI  CH1    0.10
   CAJ    C    0.35
   OAK   OM   -0.75
   OAL   OM   -0.75
   CAM  CH2    0.04
   CAN    C    0.37
   OAO   OM   -0.70
   OAP   OM   -0.70
[ bonds ]
   CAM -CAG
   CAA  CAB
   CAB  CAC
   CAC  CAD
   CAD  CAE
   CAE  CAF
   CAF  CAG
   CAG  CAH
   CAH  CAI
   CAI  CAJ
   CAI  CAM
   CAJ  OAK
   CAJ  OAL
   CAM  CAN
   CAN  OAO
   CAN  OAP
   CAG +CAM
 [ impropers ]
   CAG  CAF -CAM  C
  -CAM -CAN -CAI  C
   CAJ  CAI  OAL  O
   CAN  CAM  OAP  O
   CAI  CAH  CAJ  C

[ DR0 ]   ;head of
 [ atoms ]
   CA1  CH3    0.00
   CA2  CH2    0.00
   CA3  CH2    0.00
   CA4  CH2    0.00
   CA5  CH2    0.00
   CA6  CH2    0.00
   CA7  CH2    0.00
   CA8  CH2    0.04
   CA9  CH1    0.10
   C10    C    0.35
   O12   OM   -0.75
   O11   OM   -0.75
   C13  CH2    0.04
   C14    C    0.37
   O16   OM   -0.70
   O15   OM   -0.70
[ bonds ]
   CA1  CA2
   CA2  CA3
   CA3  CA4
   CA4  CA5
   CA5  CA6
   CA6  CA7
   CA7  CA8
   CA8  CA9
   CA9  C10
   CA9  C13
   C10  O12
   C10  O11
   C13  C14
   C14  O16
   C14  O15
   CA7 +CAM
 [ impropers ]
 CA7  CA6 +CAM  CA8
+CAM +CAI +CAN  CA7
 C10  CA9  O11  O12
 C14  C13  O15  O16
 CA9  CA8  C10  C13

[ DR1 ] ; tail of t
   CF1  CH3    0.00
   CF2  CH2    0.00
   CF3  CH2    0.00
   CF4  CH2    0.00
   CF5  CH2    0.00
   CF6  CH2    0.00
   CF7  CH2    0.00
   CF8  CH2    0.04
   CF9  CH1    0.10
   C1F    C    0.35
   O3F   OM   -0.75
   O2F   OM   -0.75
   C4F  CH2    0.04
   C5F    C    0.37
   O7F   OM   -0.70
   O6F   OM   -0.70
[ bonds ]
   C4F -CAG
   CF1  CF2
   CF2  CF3
   CF3  CF4
   CF4  CF5
   CF5  CF6
   CF6  CF7
   CF7  CF8
   CF8  CF9
   CF9  C1F
   CF9  C4F
   C1F  O3F
   C1F  O2F
   C4F  C5F
   C5F  O7F
   C5F  O6F
 [ impropers ]
 C4F  C5F  CF9 -CAG
-CAG -CAF -CAH  C4F
 C1F  CF9  O2F  O3F
 C5F  C4F  O6F  O7F
 CF9  CF8  C1F  C4F


--------------------------------------------------
From: "Justin A. Lemkul" <jalemkul at vt.edu>
Sent: Wednesday, August 12, 2009 9:50 PM
To: "Gromacs Users' List" <gmx-users at gromacs.org>
Subject: Re: [gmx-users] bad bond in polymer

>
>
> nicegromacs wrote:
>
>>   CAG -CAM
>
> Defining a bond to a previous residue will certainly fail for at least one 
> terminus of your polymer chain.  You may find this post useful:
>
> http://oldwww.gromacs.org/pipermail/gmx-users/2009-March/040125.html
>
> If things still aren't working, please post the structure file, as well, 
> and identify the bond that is being formed that shouldn't.
>
> -Justin
>
> -- 
> ========================================
>
> Justin A. Lemkul
> Ph.D. Candidate
> ICTAS Doctoral Scholar
> Department of Biochemistry
> Virginia Tech
> Blacksburg, VA
> jalemkul[at]vt.edu | (540) 231-9080
> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>
> ========================================
> _______________________________________________
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