[gmx-users] Re: gmx-users Digest, Vol 67, Issue 60

pawan raghav pwnrghv at gmail.com
Tue Nov 10 09:03:24 CET 2009


Dear,

Your tutorial for windows are lacking of proper guidence. You should post
the steps to use it to make it more user friendly.

As I have tried from last two months but after installing it I really don't
know to execute commands on windows. If you can guide me then why don't you
not tell me the steps for using it on windows.


On 11/10/09, gmx-users-request at gromacs.org <gmx-users-request at gromacs.org>
wrote:
>
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> Today's Topics:
>
>   1. Re: Missing H from residue (Jack Shultz)
>   2. Re: Missing H from residue (Justin A. Lemkul)
>   3. Re: Missing H from residue (Jack Shultz)
>   4. Please tell me how to simulate two structures together    in a
>      water box (radhika jaswal)
>   5. (no subject) (pawan raghav)
>   6. Re: (no subject) (Mark Abraham)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Mon, 9 Nov 2009 21:38:23 -0500
> From: Jack Shultz <js at drugdiscoveryathome.com>
> Subject: Re: [gmx-users] Missing H from residue
> To: jalemkul at vt.edu, Discussion list for GROMACS users
>        <gmx-users at gromacs.org>
> Cc: Andrey Voronkov <av at drugdiscoveryathome.com>
> Message-ID:
>        <6c65435e0911091838x89cddeera26061500376e186 at mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> On Mon, Nov 9, 2009 at 8:35 PM, Justin A. Lemkul <jalemkul at vt.edu> wrote:
> >
> >
> > Jack Shultz wrote:
> >
> > <snip>
> >
> >> -------------------------------------------------------
> >> Program pdb2gmx, VERSION 4.0.5
> >> Source code file: pdb2gmx.c, line: 429
> >>
> >> Fatal error:
> >> Atom O in residue CARG 244 not found in rtp entry with 25 atoms
> >>             while sorting atoms
> >> -------------------------------------------------------
> >>
> >> But we got an O here
> >> ATOM   3822  O   CARG  153      -4.455 -21.080   2.791  1.00  0.00
> >>   O
> >>
> >
> > The error is not telling you that the O atom is missing from the .pdb
> file;
> > it is telling you that it is present in the .pdb, but not in the .rtp
> entry.
> >  Look at the .rtp entry for CARG
>
> [ CARG ]
> [ atoms ]
>     N    amber99_34  -0.34810     1
>     H    amber99_17   0.27640     2
>    CA    amber99_11  -0.30680     3
>    HA    amber99_19   0.14470     4
>    CB    amber99_11  -0.03740     5
>   HB1    amber99_18   0.03710     6
>   HB2    amber99_18   0.03710     7
>    CG    amber99_11   0.07440     8
>   HG1    amber99_18   0.01850     9
>   HG2    amber99_18   0.01850    10
>    CD    amber99_11   0.11140    11
>   HD1    amber99_19   0.04680    12
>   HD2    amber99_19   0.04680    13
>    NE    amber99_38  -0.55640    14
>    HE    amber99_17   0.34790    15
>    CZ    amber99_3    0.83680    16
>   NH1    amber99_38  -0.87370    17
> HH11    amber99_17   0.44930    18
> HH12    amber99_17   0.44930    19
>   NH2    amber99_38  -0.87370    20
> HH21    amber99_17   0.44930    21
> HH22    amber99_17   0.44930    22
>     C    amber99_2    0.85570    23
>   OC1    amber99_45  -0.82660    24
>   OC2    amber99_45  -0.82660    25
>
> - you should have an O1 and O2 atom for the
>
> I made the following modification
> ATOM   1900  O1  CARG  153     -17.391
> -20.267  11.508  1.00  0.00           O
> ATOM   1909  O2  CARG  153     -18.666
> -19.390  13.107  1.00  0.00           O
>
>
> -------------------------------------------------------
> Program pdb2gmx, VERSION 4.0.5
> Source code file: pdb2gmx.c, line: 429
>
> Fatal error:
> Atom O in residue CARG 122 not found in rtp entry with 25 atoms
>             while sorting atoms
> -------------------------------------------------------
>
>
> > C-terminal carboxylate.
> >
> > -Justin
> >
> > --
> > ========================================
> >
> > Justin A. Lemkul
> > Ph.D. Candidate
> > ICTAS Doctoral Scholar
> > Department of Biochemistry
> > Virginia Tech
> > Blacksburg, VA
> > jalemkul[at]vt.edu | (540) 231-9080
> > http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> >
> > ========================================
> > --
> > gmx-users mailing list    gmx-users at gromacs.org
> > http://lists.gromacs.org/mailman/listinfo/gmx-users
> > Please search the archive at http://www.gromacs.org/search before
> posting!
> > Please don't post (un)subscribe requests to the list. Use the www
> interface
> > or send it to gmx-users-request at gromacs.org.
> > Can't post? Read http://www.gromacs.org/mailing_lists/users.php
> >
>
>
>
> --
> Jack
>
> http://drugdiscoveryathome.com
> http://hydrogenathome.org
>
>
> ------------------------------
>
> Message: 2
> Date: Mon, 09 Nov 2009 22:00:05 -0500
> From: "Justin A. Lemkul" <jalemkul at vt.edu>
> Subject: Re: [gmx-users] Missing H from residue
> To: "Gromacs Users' List" <gmx-users at gromacs.org>
> Message-ID: <4AF8D735.1030707 at vt.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
>
>
> Jack Shultz wrote:
> > On Mon, Nov 9, 2009 at 8:35 PM, Justin A. Lemkul <jalemkul at vt.edu>
> wrote:
> >>
> >> Jack Shultz wrote:
> >>
> >> <snip>
> >>
> >>> -------------------------------------------------------
> >>> Program pdb2gmx, VERSION 4.0.5
> >>> Source code file: pdb2gmx.c, line: 429
> >>>
> >>> Fatal error:
> >>> Atom O in residue CARG 244 not found in rtp entry with 25 atoms
> >>>             while sorting atoms
> >>> -------------------------------------------------------
> >>>
> >>> But we got an O here
> >>> ATOM   3822  O   CARG  153      -4.455 -21.080   2.791  1.00  0.00
> >>>   O
> >>>
> >> The error is not telling you that the O atom is missing from the .pdb
> file;
> >> it is telling you that it is present in the .pdb, but not in the .rtp
> entry.
> >>  Look at the .rtp entry for CARG
> >
> > [ CARG ]
> >  [ atoms ]
> >      N    amber99_34  -0.34810     1
> >      H    amber99_17   0.27640     2
> >     CA    amber99_11  -0.30680     3
> >     HA    amber99_19   0.14470     4
> >     CB    amber99_11  -0.03740     5
> >    HB1    amber99_18   0.03710     6
> >    HB2    amber99_18   0.03710     7
> >     CG    amber99_11   0.07440     8
> >    HG1    amber99_18   0.01850     9
> >    HG2    amber99_18   0.01850    10
> >     CD    amber99_11   0.11140    11
> >    HD1    amber99_19   0.04680    12
> >    HD2    amber99_19   0.04680    13
> >     NE    amber99_38  -0.55640    14
> >     HE    amber99_17   0.34790    15
> >     CZ    amber99_3    0.83680    16
> >    NH1    amber99_38  -0.87370    17
> >   HH11    amber99_17   0.44930    18
> >   HH12    amber99_17   0.44930    19
> >    NH2    amber99_38  -0.87370    20
> >   HH21    amber99_17   0.44930    21
> >   HH22    amber99_17   0.44930    22
> >      C    amber99_2    0.85570    23
> >    OC1    amber99_45  -0.82660    24
> >    OC2    amber99_45  -0.82660    25
> >
> > - you should have an O1 and O2 atom for the
> >
> > I made the following modification
> > ATOM   1900  O1  CARG  153     -17.391
> -20.267  11.508  1.00  0.00           O
> > ATOM   1909  O2  CARG  153     -18.666
> -19.390  13.107  1.00  0.00           O
> >
>
> Forgive my typo, what I meant was OC1 and OC2.  You must always make sure
> the
> atoms in the structure file match those expected by the .rtp file.  You
> need OC1
> and OC2.
>
> -Justin
>
> >
> > -------------------------------------------------------
> > Program pdb2gmx, VERSION 4.0.5
> > Source code file: pdb2gmx.c, line: 429
> >
> > Fatal error:
> > Atom O in residue CARG 122 not found in rtp entry with 25 atoms
> >              while sorting atoms
> > -------------------------------------------------------
> >
> >
> >> C-terminal carboxylate.
> >>
> >> -Justin
> >>
> >> --
> >> ========================================
> >>
> >> Justin A. Lemkul
> >> Ph.D. Candidate
> >> ICTAS Doctoral Scholar
> >> Department of Biochemistry
> >> Virginia Tech
> >> Blacksburg, VA
> >> jalemkul[at]vt.edu | (540) 231-9080
> >> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> >>
> >> ========================================
> >> --
> >> gmx-users mailing list    gmx-users at gromacs.org
> >> http://lists.gromacs.org/mailman/listinfo/gmx-users
> >> Please search the archive at http://www.gromacs.org/search before
> posting!
> >> Please don't post (un)subscribe requests to the list. Use the www
> interface
> >> or send it to gmx-users-request at gromacs.org.
> >> Can't post? Read http://www.gromacs.org/mailing_lists/users.php
> >>
> >
> >
> >
>
> --
> ========================================
>
> Justin A. Lemkul
> Ph.D. Candidate
> ICTAS Doctoral Scholar
> Department of Biochemistry
> Virginia Tech
> Blacksburg, VA
> jalemkul[at]vt.edu | (540) 231-9080
> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
>
> ========================================
>
>
> ------------------------------
>
> Message: 3
> Date: Mon, 9 Nov 2009 22:10:22 -0500
> From: Jack Shultz <js at drugdiscoveryathome.com>
> Subject: Re: [gmx-users] Missing H from residue
> To: jalemkul at vt.edu, Discussion list for GROMACS users
>        <gmx-users at gromacs.org>
> Message-ID:
>        <6c65435e0911091910r7c932c23l2a2814b72d8be69a at mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Thanks Justin! Got it now.
>
> On Mon, Nov 9, 2009 at 10:00 PM, Justin A. Lemkul <jalemkul at vt.edu> wrote:
> >
> >
> > Jack Shultz wrote:
> >>
> >> On Mon, Nov 9, 2009 at 8:35 PM, Justin A. Lemkul <jalemkul at vt.edu>
> wrote:
> >>>
> >>> Jack Shultz wrote:
> >>>
> >>> <snip>
> >>>
> >>>> -------------------------------------------------------
> >>>> Program pdb2gmx, VERSION 4.0.5
> >>>> Source code file: pdb2gmx.c, line: 429
> >>>>
> >>>> Fatal error:
> >>>> Atom O in residue CARG 244 not found in rtp entry with 25 atoms
> >>>>            while sorting atoms
> >>>> -------------------------------------------------------
> >>>>
> >>>> But we got an O here
> >>>> ATOM   3822  O   CARG  153      -4.455 -21.080   2.791  1.00  0.00
> >>>>  O
> >>>>
> >>> The error is not telling you that the O atom is missing from the .pdb
> >>> file;
> >>> it is telling you that it is present in the .pdb, but not in the .rtp
> >>> entry.
> >>>  Look at the .rtp entry for CARG
> >>
> >> [ CARG ]
> >>  [ atoms ]
> >>     N    amber99_34  -0.34810     1
> >>     H    amber99_17   0.27640     2
> >>    CA    amber99_11  -0.30680     3
> >>    HA    amber99_19   0.14470     4
> >>    CB    amber99_11  -0.03740     5
> >>   HB1    amber99_18   0.03710     6
> >>   HB2    amber99_18   0.03710     7
> >>    CG    amber99_11   0.07440     8
> >>   HG1    amber99_18   0.01850     9
> >>   HG2    amber99_18   0.01850    10
> >>    CD    amber99_11   0.11140    11
> >>   HD1    amber99_19   0.04680    12
> >>   HD2    amber99_19   0.04680    13
> >>    NE    amber99_38  -0.55640    14
> >>    HE    amber99_17   0.34790    15
> >>    CZ    amber99_3    0.83680    16
> >>   NH1    amber99_38  -0.87370    17
> >>  HH11    amber99_17   0.44930    18
> >>  HH12    amber99_17   0.44930    19
> >>   NH2    amber99_38  -0.87370    20
> >>  HH21    amber99_17   0.44930    21
> >>  HH22    amber99_17   0.44930    22
> >>     C    amber99_2    0.85570    23
> >>   OC1    amber99_45  -0.82660    24
> >>   OC2    amber99_45  -0.82660    25
> >>
> >> - you should have an O1 and O2 atom for the
> >>
> >> I made the following modification
> >> ATOM   1900  O1  CARG  153     -17.391 -20.267  11.508  1.00  0.00
> >>   O
> >> ATOM   1909  O2  CARG  153     -18.666 -19.390  13.107  1.00  0.00
> >>   O
> >>
> >
> > Forgive my typo, what I meant was OC1 and OC2.  You must always make sure
> > the atoms in the structure file match those expected by the .rtp
> file.  You
> > need OC1 and OC2.
> >
> > -Justin
> >
> >>
> >> -------------------------------------------------------
> >> Program pdb2gmx, VERSION 4.0.5
> >> Source code file: pdb2gmx.c, line: 429
> >>
> >> Fatal error:
> >> Atom O in residue CARG 122 not found in rtp entry with 25 atoms
> >>             while sorting atoms
> >> -------------------------------------------------------
> >>
> >>
> >>> C-terminal carboxylate.
> >>>
> >>> -Justin
> >>>
> >>> --
> >>> ========================================
> >>>
> >>> Justin A. Lemkul
> >>> Ph.D. Candidate
> >>> ICTAS Doctoral Scholar
> >>> Department of Biochemistry
> >>> Virginia Tech
> >>> Blacksburg, VA
> >>> jalemkul[at]vt.edu | (540) 231-9080
> >>> http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> >>>
> >>> ========================================
> >>> --
> >>> gmx-users mailing list    gmx-users at gromacs.org
> >>> http://lists.gromacs.org/mailman/listinfo/gmx-users
> >>> Please search the archive at http://www.gromacs.org/search before
> >>> posting!
> >>> Please don't post (un)subscribe requests to the list. Use the www
> >>> interface
> >>> or send it to gmx-users-request at gromacs.org.
> >>> Can't post? Read http://www.gromacs.org/mailing_lists/users.php
> >>>
> >>
> >>
> >>
> >
> > --
> > ========================================
> >
> > Justin A. Lemkul
> > Ph.D. Candidate
> > ICTAS Doctoral Scholar
> > Department of Biochemistry
> > Virginia Tech
> > Blacksburg, VA
> > jalemkul[at]vt.edu | (540) 231-9080
> > http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> >
> > ========================================
> > --
> > gmx-users mailing list    gmx-users at gromacs.org
> > http://lists.gromacs.org/mailman/listinfo/gmx-users
> > Please search the archive at http://www.gromacs.org/search before
> posting!
> > Please don't post (un)subscribe requests to the list. Use the www
> interface
> > or send it to gmx-users-request at gromacs.org.
> > Can't post? Read http://www.gromacs.org/mailing_lists/users.php
> >
>
>
>
> --
> Jack
>
> http://drugdiscoveryathome.com
> http://hydrogenathome.org
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 10 Nov 2009 12:10:57 +0530 (IST)
> From: radhika jaswal <jaswalradhika at yahoo.co.in>
> Subject: [gmx-users] Please tell me how to simulate two structures
>        together        in a water box
> To: gmx-users at gromacs.org
> Message-ID: <864176.49303.qm at web94808.mail.in2.yahoo.com>
> Content-Type: text/plain; charset="utf-8"
>
> Respected Sir,
>
> Please tell me how to simulate two different stuctures together in a
> water box so as to have an idea of drug docking and what options to
> use. Any suggestions are welcome.
>
>
>
> With Regards
>
> Radhika
>
>
>
>
>
>
>      Connect more, do more and share more with Yahoo! India Mail. Learn
> more. http://in.overview.mail.yahoo.com/
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> ------------------------------
>
> Message: 5
> Date: Tue, 10 Nov 2009 12:16:56 +0530
> From: pawan raghav <pwnrghv at gmail.com>
> Subject: [gmx-users] (no subject)
> To: gmx-users at gromacs.org
> Message-ID:
>        <c7ae933b0911092246he5f982fyad982886dfe5cb6c at mail.gmail.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> After installing gromacs4.0 on windows it created Gromacs directory
> containing several  .exe files in bin directory. But when I used pdb2gmx
> command it will shows that basch command is not found. So anyone can tell
> me
> what is that.
>
> --
> Pawan Kumar Raghav
> Bioinformatician
> Stem Cell and Gene Therapy Research Group
> INMAS, DRDO
> Timarpur Delhi-110054
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> ------------------------------
>
> Message: 6
> Date: Tue, 10 Nov 2009 17:59:19 +1100
> From: Mark Abraham <Mark.Abraham at anu.edu.au>
> Subject: Re: [gmx-users] (no subject)
> To: Discussion list for GROMACS users <gmx-users at gromacs.org>
> Message-ID: <4AF90F47.8030805 at anu.edu.au>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> pawan raghav wrote:
> > After installing gromacs4.0 on windows it created Gromacs directory
> > containing several  .exe files in bin directory. But when I used pdb2gmx
> > command it will shows that basch command is not found. So anyone can
> > tell me what is that.
>
> See point 8 here
>
> http://www.gromacs.org/index.php?title=Download_%26_Installation/Installation_Instructions
>
> Mark
>
>
> ------------------------------
>
> --
> gmx-users mailing list
> gmx-users at gromacs.org
> http://lists.gromacs.org/mailman/listinfo/gmx-users
> Please search the archive at http://www.gromacs.org/search before posting!
>
> End of gmx-users Digest, Vol 67, Issue 60
> *****************************************
>



-- 
Pawan Kumar Raghav
Bioinformatician
Stem Cell and Gene Therapy Research Group
INMAS, DRDO
Timarpur Delhi-110054
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