[gmx-users] charge group radii larger that rlist-rvdw

Justin A. Lemkul jalemkul at vt.edu
Sat Jan 22 18:48:16 CET 2011



Kavyashree M wrote:
> I went through few papers related to OPLS-AA, but could not find
> any specific values mentioned for vdw and coulomb intecations..
> In one of the papers
> ".....In most cases, the intermolecular nonbonded interactions were
> truncated at 11Å based on roughly the center-of-mass separations
> with quadratic smoothing of the interaction energy to zero over the
> last 0.5 Å. The cutoff for alkenes and acetals was 13 Å, while for
> propanol, cyclohexane, 2-methyl-2-propanol, phenol, ethyl methyl
> sulfide, NMA, DMA, and NMP it was extended to 15 Å....."
> J. Am. Chem. Soc. 1996, 118, 11225-11236.
> 

That is an older version of OPLS.  See dx.doi.org/10.1021/jp003919d.  The 
cutoffs are much clearer.

-Justin

> 
> So does this cut off refer to coulombic interactions?
> is it correct if iI use the following parameters for OPLS-AA force field?
> 
> -----------------------------------------------------------------------------
> ;NEIGHBOUR SEARCHING
> nstlist         = 10                    ; FREQUENCY WITH WHICH 
> NEIGHBOURLIST IS UPDATED
> ns_type         = grid                  ; TYPE OF NEIGHBOUR SEARCH GRID 
> OR SIMPLE
> pbc             = xyz                   ; DIRECTION OF PERIODIC BOUNDARY 
> CONDITIONS USAGE
> rlist           = 1.4                  ; CUT-OFF DISTANCE FOR SHORT 
> RANGE NEIGHBOUR LIST
> 
> ;ELECTROSTATICS
> coulombtype     = PME                   ; METHOD FOR CALCULATING 
> COULOMIC INTERCATION - PARTICLE MESH EWALD
> rcoulomb        = 1.4                  ; CUTOFF DISTANCE FOR 
> ELECTROSTATIC INTERACTIONS
> epsilon_r       = 1                     ; RELATIVE DIELECTRIC CONSTANT
> 
> ;VAN DER WAALS
> vdwtype         = Switch                ; METHOD FOR TREATING 
> VANDERWAAL'S FORCES
> rvdw_switch     = 0.9
> rvdw            = 1.00                  ; CUTOFF DISTANCE FOR LJ OR 
> BUCKINGHAM INTERACTIONS
> --------------------------------------------------------------------------------------------------------------------
> 
> 
> Thanks
> MKS
> 
> 
> On Sat, Jan 22, 2011 at 6:41 PM, Justin A. Lemkul <jalemkul at vt.edu 
> <mailto:jalemkul at vt.edu>> wrote:
> 
> 
> 
>     Kavyashree M wrote:
> 
>         Sir,
> 
>             Yes sir I am using PME. And one more thing I noticed was,
>         the protein has
>         7 disulphide bonds, so when I reduce all the cystine to cysteine
>         or to alanine,
>         the the sum of charge group radii reduces from 0.31..nm to
>         0.30..nm and also the
>         value of this is identical in case of alanine mutation or
>         cysteine mutation.
>            I finalised on the value of rlist = rcolomb = 1.35nm so as
>         not to take risk.
>         is that fine?
>          
> 
> 
>     As I said before, read the OPLS paper.  1.35 nm does not sound right.
> 
>     -Justin
> 
>         Thank you
>         M. Kavyashree
> 
> 
>         On Sat, Jan 22, 2011 at 6:26 PM, Justin A. Lemkul
>         <jalemkul at vt.edu <mailto:jalemkul at vt.edu>
>         <mailto:jalemkul at vt.edu <mailto:jalemkul at vt.edu>>> wrote:
> 
> 
> 
>            Kavyashree M wrote:
> 
>                Sir,
> 
>                    System is a protein with 123 aa. No modifications
>         were made
>                on the pdb file,
>                and it was submitted to pdb2gmx by removing all HETATMs,
>                retaining only protein
>                atoms. topology was created entirely by pdb2gmx and no other
>                molecule was introduced.
> 
>                    I will go through the OPLSAA paper, but if I increase
>         rlist
>                to 1.4nm without reducing
>                rvdw according to "The Origin of Layer Structure Artifacts in
>                Simulations of Liquid Water"
>                - JCTC, 2006, 2, 1-11, will it not cause orderedwater shell
>                during simultion?
> 
> 
>            You're using PME.  In the first paragraph of the
>         introduction, it is
>            stated that the spurious effect does not occur with PME.
> 
>            -Justin
> 
> 
>                Thanking you
>                M. Kavyashree
> 
> 
>                On Sat, Jan 22, 2011 at 12:27 AM, Justin A. Lemkul
>                <jalemkul at vt.edu <mailto:jalemkul at vt.edu>
>         <mailto:jalemkul at vt.edu <mailto:jalemkul at vt.edu>>
>                <mailto:jalemkul at vt.edu <mailto:jalemkul at vt.edu>
>         <mailto:jalemkul at vt.edu <mailto:jalemkul at vt.edu>>>> wrote:
> 
> 
> 
>                   Kavyashree M wrote:
> 
>                       Sir,
> 
>                         What exactly can be wrong with the topology? As
>         I tried
>                with
> 
> 
>                   I don't know, what's in your system?  Was the topology
>         created
>                   entirely by pdb2gmx, or have you introduced some other
>         molecules
>                   that you've parameterized?
> 
> 
>                       different PDBs of same structure at higher resolutions
>                too, I am
>                       getting
>                       almost similar charge group radii, so can you kindly
>                elaborate about
>                       what can go wrong in the topology to get such values?
> 
> 
>                   Large charge groups indicate that a fair number of
>         atoms have
>                been
>                   included in the same charge group.  Usually only two
>         or three
>                atoms
>                   are in a charge group, rendering them fairly small.  With
>                PME, the
>                   effects may not be that large, i.e.
>                        
>          http://lists.gromacs.org/pipermail/gmx-users/2010-November/056219.html,
>                   but it's still worth investigating what your largest
>         charge
>                group is
>                   that's causing this problem.  If the topology is sound
>         and is
>                based
>                   entirely upon standard force field files, it may
>         suffice to
>                simply
>                   increase rlist to 1.4 nm, leaving all the other
>         cutoffs at 1.0,
>                   which I believe is standard for OPLS (but don't just
>         take my word
>                   for it).
> 
>                   -Justin
> 
> 
>                   Justin A. Lemkul
>                   Ph.D. Candidate
>                   ICTAS Doctoral Scholar
>                   MILES-IGERT Trainee
>                   Department of Biochemistry
>                   Virginia Tech
>                   Blacksburg, VA
>                   jalemkul[at]vt.edu <http://vt.edu> <http://vt.edu>
>         <http://vt.edu> | (540)
> 
>                231-9080
> 
>                   http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> 
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>            --     ========================================
> 
> 
>            Justin A. Lemkul
>            Ph.D. Candidate
>            ICTAS Doctoral Scholar
>            MILES-IGERT Trainee
>            Department of Biochemistry
>            Virginia Tech
>            Blacksburg, VA
>            jalemkul[at]vt.edu <http://vt.edu> <http://vt.edu> | (540)
>         231-9080
>            http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> 
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>     -- 
>     ========================================
> 
>     Justin A. Lemkul
>     Ph.D. Candidate
>     ICTAS Doctoral Scholar
>     MILES-IGERT Trainee
>     Department of Biochemistry
>     Virginia Tech
>     Blacksburg, VA
>     jalemkul[at]vt.edu <http://vt.edu> | (540) 231-9080
>     http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
> 
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-- 
========================================

Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
MILES-IGERT Trainee
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

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