[gmx-users] want to remove H5T entry form HDB file / open terminal (P, O) for polymerization of i-motif

Singh raghavbioinfo at gmail.com
Thu Jul 7 16:56:40 CEST 2011


Hi Friends,

could anyone please guide me to get rid of the 5' H atom in terminal DT nucleotide. These are my entries..

PDB : 5'DT
ATOM      1  P    DT A   1      -6.726  -4.074 -28.509  1.00  0.00           P  
ATOM      2  O5'  DT A   1      -5.444  -3.882 -27.274  1.00  0.00           O1-
ATOM      3  C5'  DT A   1      -4.975  -5.193 -26.943  1.00  0.00           C  
ATOM      4 1H5'  DT A   1      -4.594  -5.667 -27.848  1.00  0.00           H  
ATOM      5 2H5'  DT A   1      -5.809  -5.781 -26.560  1.00  0.00           H  
ATOM      6  C4'  DT A   1      -3.861  -5.152 -25.898  1.00  0.00           C  
ATOM      7  H4'  DT A   1      -3.531  -6.170 -25.685  1.00  0.00           H  
ATOM      8  O4'  DT A   1      -2.738  -4.387 -26.383  1.00  0.00           O  
ATOM      9  C1'  DT A   1      -2.272  -3.512 -25.342  1.00  0.00           C  
ATOM     10  H1'  DT A   1      -1.204  -3.694 -25.215  1.00  0.00           H  
ATOM     11  N1   DT A   1      -2.478  -2.073 -25.683  1.00  0.00           N  
ATOM     12  C6   DT A   1      -3.733  -1.556 -25.872  1.00  0.00           C  
ATOM     13  H6   DT A   1      -4.556  -2.210 -26.150  1.00  0.00           H  
ATOM     14  C2   DT A   1      -1.358  -1.256 -25.798  1.00  0.00           C  
ATOM     15  O2   DT A   1      -0.205  -1.685 -25.775  1.00  0.00           O  
ATOM     16  N3   DT A   1      -1.613   0.096 -25.966  1.00  0.00           N  
ATOM     17  H3   DT A   1      -0.819   0.705 -26.089  1.00  0.00           H  
ATOM     18  C4   DT A   1      -2.857   0.699 -25.989  1.00  0.00           C  
ATOM     19  O4   DT A   1      -2.954   1.918 -26.111  1.00  0.00           O  
ATOM     20  C5   DT A   1      -3.955  -0.231 -25.855  1.00  0.00           C  
ATOM     21  C7   DT A   1      -5.368   0.283 -25.580  1.00  0.00           C  
ATOM     22  H71  DT A   1      -5.381   1.373 -25.594  1.00  0.00           H  
ATOM     23  H72  DT A   1      -5.685  -0.067 -24.597  1.00  0.00           H  
ATOM     24  H73  DT A   1      -6.051  -0.098 -26.340  1.00  0.00           H  
ATOM     25  C2'  DT A   1      -2.985  -4.008 -24.085  1.00  0.00           C  
ATOM     26 1H2'  DT A   1      -2.460  -4.883 -23.698  1.00  0.00           H  
ATOM     27 2H2'  DT A   1      -3.043  -3.243 -23.310  1.00  0.00           H  
ATOM     28  C3'  DT A   1      -4.324  -4.508 -24.586  1.00  0.00           C  
ATOM     29  H3'  DT A   1      -4.981  -3.657 -24.744  1.00  0.00           H  
ATOM     30  O3'  DT A   1      -4.947  -5.483 -23.741  1.00  0.00           O  
ATOM     31  O1P  DT A   1      -7.256  -2.725 -28.895  1.00  0.00           O1-  
ATOM     32  O2P  DT A   1      -7.840  -4.907 -27.948  1.00  0.00           O1-

RTP Entry : DT5
[ DT5 ]
 [ atoms ]
   H5T    HO            0.44220     1
   O5'    O            -0.63180     2
   C5'    CT           -0.00690     3
  H5'1    H1            0.07540     4
  H5'2    H1            0.07540     5
   C4'    CT            0.16290     6
   H4'    H1            0.11760     7
   O4'    OS           -0.36910     8
   C1'    CT            0.06800     9
   H1'    H2            0.18040    10
    N1    N*           -0.02390    11
    C6    CM           -0.22090    12
    H6    H4            0.26070    13
    C5    CM            0.00250    14
    C7    CT           -0.22690    15
   H71    HC            0.07700    16
   H72    HC            0.07700    17
   H73    HC            0.07700    18
    C4    C             0.51940    19
    O4    O            -0.55630    20
    N3    NA           -0.43400    21
    H3    H             0.34200    22
    C2    C             0.56770    23
    O2    O            -0.58810    24
   C3'    CT            0.07130    25
   H3'    H1            0.09850    26
   C2'    CT           -0.08540    27
  H2'1    HC            0.07180    28
  H2'2    HC            0.07180    29
   O3'    OS           -0.52320    30
   P      P             1.16590    31
   O1P    O2           -0.77610    32
   O2P    O2           -0.77610    33
                  
 [ bonds ]
   O1P   P
   O2P   P
   O3P   H5T
   O5'   P
   O5'   C5'
   C5'  H5'1
   C5'  H5'2
   C5'   C4'
   C4'   H4'
   C4'   O4'
   C4'   C3'
   O4'   C1'
   C1'   H1'
   C1'    N1
   C1'   C2'
    N1    C6
    N1    C2
    C6    H6
    C6    C5
    C5    C7
    C5    C4
    C7   H71
    C7   H72
    C7   H73
    C4    O4
    C4    N3
    N3    H3
    N3    C2
    C2    O2
   C3'   H3'
   C3'   C2'
   C3'   O3'
   C2'  H2'1
   C2'  H2'2
 [ impropers ]
    C2    C6    N1   C1'
    C4    C6    C5    C7
    N1    N3    C2    O2
    C5    N3    C4    O4
    C4    C2    N3    H3  
    N1    C5    C6    H6

HDB Entry for DT5 (modified)

DT5	9
1	2	H5T	 	O2P	 P	 	O5'	
2	6	H5'	 	C5'	        O5'	 	C4'	
1	5	H4'	 	C4'	        C5'	 	O4'	 C3'	
1	5	H1' 	 	C1'	 	O4'	 	N1	 C2'	
1	1	H6	 	C6	 	N1	 	C5	
3	4	H7 	 	C7	 	C5	 	C6	
1	1	H3		 N3	 	C4 		C2	
1	5	H3'		 C3'	 	C4'	 	C2'	O3'	
2	6	H2' 	 	C2'	 	C1'	 	C3'	


I am trying a lot to solve this problem .. but Unfortunately I am not able.. :( .. Please have a look and if possible make some suggestions on RTP or  HDB entry !!

Raghav
Research Scholar
IEMN, University of Science and Technology
Villeneuve d'ascq, Lille, France







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