[gmx-users] Gromos 53a6, .mdp parameters for reaction field

Mohsen Ramezanpour ramezanpour.mohsen at gmail.com
Wed Jul 22 00:44:26 CEST 2015


I looked at the first link, it was a useful discussion.
Now, I am trying suggestions with rlist/r(columb/vdw)=(1.4/1.4) and
everything is working properly till now.
It seems the problem was the "twin-range" + RF with this version of Gromacs
as has been discussed and you mentioned already.

I am playing a little bit to see if I everything will be fine with the rest
of test simulation.
I will reply again after my tests.

Thanks for the links

Cheers
Mohsen

On Tue, Jul 21, 2015 at 4:21 PM, João M. Damas <jmdamas at itqb.unl.pt> wrote:

> I really think you may be losing time with LINCS parameters. Look at these
> links too:
>
>
> https://mailman-1.sys.kth.se/pipermail/gromacs.org_gmx-users/2014-March/088108.html
> http://redmine.gromacs.org/issues/1400
>
> You use a twin-range, i.e. a rlist/r(coulomb/vdw) of 0.8/1.4, so I really
> think that this may be the source of your problems. The links that I sent
> you present solutions to your problem, but almost always at a computational
> cost, i.e. slower simulations.
>
> João
>
>
> On Tue, Jul 21, 2015 at 9:34 PM, V.V.Chaban <vvchaban at gmail.com> wrote:
>
> > Maybe indeed downgrade to an earlier version.
> >
> > I, for sure, successfully used reaction field with gromacs 3-3-3
> > (imagine when it was).
> >
> > Professor Vitaly V. Chaban
> >
> >
> >
> >
> > On Tue, Jul 21, 2015 at 4:49 PM, Mohsen Ramezanpour
> > <ramezanpour.mohsen at gmail.com> wrote:
> > > Hi Chaban,
> > >
> > > Me too, but I do not know why starting with the same initial structures
> > and
> > > also starting from equilibrated structures again cause LINCS problems
> in
> > one
> > > not in the other one.
> > >
> > > I have tried lincs-iter and lincs-order and also lincs allowable angle
> > > deviations but LINCS error still exist. This is why I think there is
> > > something wrong with other parameters, e.g. reaction field!
> > > Sure, I will look at epsilon_r, thanks.
> > >
> > >
> > > On Tue, Jul 21, 2015 at 11:44 AM, V.V.Chaban <vvchaban at gmail.com>
> wrote:
> > >>
> > >> I do not see much connection between bond constraints and treatment of
> > >> electrostatics beyond the cut-off distance.
> > >>
> > >> There is also an "epsilon_r" keyword. I would investigate what exactly
> > >> it means and whether it is used in the reaction field procedure.
> > >>
> > >> The "all-bonds" contraint often results in different problems. You
> > >> will have to play with the relevant parameters, like lincs_order and
> > >> allowable deviation.
> > >>
> > >>
> > >>
> > >>
> > >>
> > >>
> > >> On Tue, Jul 21, 2015 at 2:28 PM, Mohsen Ramezanpour
> > >> <ramezanpour.mohsen at gmail.com> wrote:
> > >> > Dear Gromacs users,
> > >> >
> > >> > I am interested in simulation of lipid bilayers with Gromos96 53a6.
> > >> > As I understood this force field has been parametrized with reaction
> > >> > field
> > >> > instead of pme.
> > >> >
> > >> > Based on literatures, I have the following .mdp parameters:
> > >> > But I am not sure of those as my system has lots of LINCS warnings
> > with
> > >> > these parameters.
> > >> >
> > >> > integrator               = md
> > >> > tinit                       = 0
> > >> > dt                          = 0.002
> > >> > nsteps                   = 50000
> > >> > nstcomm               = 1
> > >> > comm-grps            =
> > >> >
> > >> >
> > >> > nstxout                  = 0
> > >> > nstvout                  = 0
> > >> > nstfout                   = 0
> > >> > nstenergy                = 100
> > >> > nstxtcout                = 5000
> > >> > xtc-precision           = 1000
> > >> > energygrps              = system
> > >> >
> > >> > nstlist                      = 5
> > >> > ns_type                   = grid
> > >> > pbc                         = xyz
> > >> > rlist                         = 0.8
> > >> >
> > >> > coulombtype             = reaction_field
> > >> > rcoulomb-switch        = 0
> > >> > rcoulomb                  = 1.4
> > >> > epsilon_rf                 = 62
> > >> > vdw-type                  = Cut-off
> > >> > rvdw-switch              = 0
> > >> > rvdw                        = 1.4
> > >> > DispCorr                  = no
> > >> >
> > >> >
> > >> > tcoupl                     = v-rescale
> > >> > tc_grps                   = lipid   water_ion
> > >> > tau_t                      =    0.1  0.1
> > >> > ref_t                       =    353  353
> > >> > Pcoupl                   = berendsen
> > >> > pcoupltype             = semiisotropic
> > >> > tau_p                     = 1.0  1.0
> > >> > compressibility       = 4.6e-5  4.6e-5
> > >> > ref_p                     = 1.0  1.0
> > >> >
> > >> >
> > >> > gen_vel                    = yes
> > >> > gen_temp                 = 353
> > >> > gen_seed                 = 17352
> > >> >
> > >> > constraints               = all-bonds
> > >> > constraint-algorithm     = Lincs
> > >> > unconstrained-start      = no
> > >> > shake-tol                    = 1e-04
> > >> > morse                        = no
> > >> >
> > >> >
> > >> > Interestingly, when I use common Gromos parameters including pme, as
> > >> > follows, I do not have any LINCS problem with my system.
> > >> >
> > >> > integrator                = md
> > >> > tinit                        = 0
> > >> > dt                           = 0.002
> > >> > nsteps                   = 50000
> > >> > pbc                        = xyz
> > >> > comm-mode           = linear
> > >> > nstcomm                = 1
> > >> > comm-grps             = system
> > >> >
> > >> >
> > >> > nstxout                  = 0
> > >> > nstvout                  = 0
> > >> > nstfout                  = 0
> > >> > nstlist                   = 10
> > >> > ns_type                 = grid
> > >> > nstenergy              = 100
> > >> > nstxtcout               = 5000
> > >> > energygrps            = system
> > >> >
> > >> >
> > >> > coulombtype              = pme
> > >> > rcoulomb                    = 1.0
> > >> > rlist                            = 1.0
> > >> > vdw-type                    = cutoff
> > >> > rvdw                         = 1.0
> > >> > DispCorr                   = no
> > >> > table-extension          = 1
> > >> >
> > >> >
> > >> > tcoupl                     = v-rescale
> > >> > tc-grps                    = lipid  water_ion
> > >> > tau-t                       = 0.1   0.1
> > >> > ref-t                        = 353   353
> > >> > Pcoupl                    = berendsen
> > >> > Pcoupltype              = semiisotropic
> > >> > ref-p                        = 1.0  1.0
> > >> > tau-p                       = 1.0  1.0
> > >> > compressibility         = 4.5e-5   4.5e-5
> > >> >
> > >> >
> > >> > constraints                  = all-bonds
> > >> > constraint-algorithm     = Lincs
> > >> >
> > >> >
> > >> > gen_vel        = yes
> > >> > gen_temp    = 353
> > >> > gen_seed    = -1
> > >> >
> > >> >
> > >> > Since the initial structures are the same, I think my parameters for
> > >> > reaction field one has problem. I have also tried shorter time steps
> > >> > (even
> > >> > 0.5 fs) and also started from different initial structures but the
> > >> > problem
> > >> > still exist.
> > >> >
> > >> > I searched the mailing list but I could not find my answer
> completely.
> > >> > Please let me know if you have any idea about parameters or if you
> > have
> > >> > any
> > >> > .mdp file for reaction field (Gromos 53a6).
> > >> >
> > >> > Cheers
> > >> > Mohsen
> > >> > --
> > >> > *Rewards work better than punishment ...*
> > >> > --
> > >> > Gromacs Users mailing list
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>
> --
> João M. Damas
> PhD Student
> Protein Modelling Group
> ITQB-UNL, Oeiras, Portugal
> Tel:+351-214469613
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*Rewards work better than punishment ...*


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