[gmx-users] energy minimization problem

[kiana moghaddam]

Hi GMX Users

I 'm running MD Simulation for DNA-ligand interaction. I ran energy minimization with emtol=10, 100 and 250 in 10000 steps (steepest descent followed by the conjugate gradient algorithm). The lowest value for energy obtained for emtol=10. 
I have some questions about energy minimization step. what is suitable value for emtol in em.mdp file? how many steps is needed for energy minimization? and how do I understand the minimization is successful? 

Best Regards
Kiana