[gmx-users] topology

RAHUL SURESH drrahulsuresh at gmail.com
Wed Mar 1 20:19:04 CET 2017


Dear Justin

What is the error here?
I don't understand it.
Can you be little more precise?

On Wed, Mar 1, 2017 at 1:58 AM, Justin Lemkul <jalemkul at vt.edu> wrote:

>
>
> On 2/28/17 3:24 AM, RAHUL SURESH wrote:
>
>> This is my itp file(only a part)
>>
>> I have highlighted two errors.. there are 19 such errors.
>> If I can know the reason for the error exactly it would be of a great help
>> to me.
>>
>>
> TopolGen relies on atom ordering to guess what the proper parameters
> should be. It's pretty limited in what it can do.  A coordinate file that
> simply groups atoms by element is going to produce a totally garbage
> topology, unfortunately.
>
> -Justin
>
>
>> [ atoms ]
>> ;   nr       type  resnr residue  atom   cgnr     charge       mass
>> typeB    chargeB      massB
>>      1   opls_154      1    UNK      O      0     -0.580921
>> 15.99940
>>      2   opls_154      1    UNK      O      0     -0.635654
>> 15.99940
>>      3   opls_516      1    UNK      C      1     -0.112257
>> 12.01100
>>      4   opls_516      1    UNK      C      2     -0.163224
>> 12.01100
>>      5   opls_516      1    UNK      C      3      0.228741
>> 12.01100
>>      6   opls_516      1    UNK      C      4     -0.304756
>> 12.01100
>>      7   opls_516      1    UNK      C      5     -0.312217
>> 12.01100
>>      8   opls_145      1    UNK      C      6      0.120569
>> 12.01100
>>      9   opls_145      1    UNK      C      7      0.151897
>> 12.01100
>>     10   opls_145      1    UNK      C      8      0.231044
>> 12.01100
>>     11   opls_516      1    UNK      C      9     -0.401751
>> 12.01100
>>     12   opls_516      1    UNK      C     10     -0.403094
>> 12.01100
>>     13   opls_145      1    UNK      C     11     -0.150352
>> 12.01100
>>     14   opls_145      1    UNK      C     12      0.193129
>> 12.01100
>>     15   opls_145      1    UNK      C     13     -0.161115
>> 12.01100
>>     16   opls_516      1    UNK      C     14     -0.471874
>> 12.01100
>>     17   opls_145      1    UNK      C     15      0.100725
>> 12.01100
>>     18   opls_145      1    UNK      C     16     -0.153943
>> 12.01100
>>     19   opls_516      1    UNK      C     17     -0.330577
>> 12.01100
>>     20   opls_516      1    UNK      C     18     -0.229875
>> 12.01100
>>     21   opls_135      1    UNK      C     19     -0.229884
>> 12.01100
>>     22   opls_135      1    UNK      C     20     -0.237230
>> 12.01100
>>     23   opls_135      1    UNK      C     21     -0.404861
>> 12.01100
>>     24   opls_140      1    UNK      H     21      0.135257
>> 1.00800
>>     25   opls_140      1    UNK      H     21      0.154010
>> 1.00800
>>     26   opls_140      1    UNK     2H     21      0.142643
>> 1.00800
>>     27   opls_140      1    UNK     1H     21      0.133623
>> 1.00800
>>     28   opls_140      1    UNK     2H     21      0.137323
>> 1.00800
>>     29   opls_140      1    UNK     1H     21      0.167475
>> 1.00800
>>     30   opls_140      1    UNK     1H     21      0.135348
>> 1.00800
>>     31   opls_140      1    UNK     2H     21      0.153851
>> 1.00800
>>     32   opls_140      1    UNK     3H     21      0.138487
>> 1.00800
>>     33   opls_140      1    UNK     1H     21      0.137087
>> 1.00800
>>     34   opls_140      1    UNK     2H     21      0.146500
>> 1.00800
>>     35   opls_140      1    UNK     3H     21      0.148972
>> 1.00800
>>     36   opls_140      1    UNK      H     21      0.103605
>> 1.00800
>>     37   opls_140      1    UNK      H     21      0.127061
>> 1.00800
>>     38   opls_140      1    UNK     1H     21      0.143100
>> 1.00800
>>     39   opls_140      1    UNK     2H     21      0.137525
>> 1.00800
>>     40   opls_140      1    UNK     3H     21      0.140672
>> 1.00800
>>     41   opls_140      1    UNK      H     21      0.106416
>> 1.00800
>>     42   opls_140      1    UNK     1H     21      0.134610
>> 1.00800
>>     43   opls_140      1    UNK     2H     21      0.126890
>> 1.00800
>>     44   opls_140      1    UNK     2H     21      0.132690
>> 1.00800
>>     45   opls_140      1    UNK     1H     21      0.124796
>> 1.00800
>>     46   opls_140      1    UNK      H     21      0.371894
>> 1.00800
>>     47   opls_140      1    UNK     1H     21      0.120642
>> 1.00800
>>     48   opls_140      1    UNK     2H     21      0.117792
>> 1.00800
>>     49   opls_140      1    UNK     2H     21      0.125342
>> 1.00800
>>     50   opls_140      1    UNK     1H     21      0.122930
>> 1.00800
>>     51   opls_140      1    UNK     1H     21      0.130012
>> 1.00800
>>     52   opls_140      1    UNK     2H     21      0.129674
>> 1.00800
>>     53   opls_140      1    UNK     3H     21      0.131255
>> 1.00800
>>
>> [ bonds ]
>> ;  ai    aj funct
>>     1     5     1    ;     O     C
>>     1    10     1    ;     O     C
>>     2    14     1    ;     O     C
>>     *2    46     1    ;     O     H*
>>
>>     3     4     1    ;     C     C
>>     3     5     1    ;     C     C
>>     3     6     1    ;     C     C
>>     3    24     1    ;     C     H
>>     4     7     1    ;     C     C
>>     4     8     1    ;     C     C
>>     4    25     1    ;     C     H
>>     5    11     1    ;     C     C
>>     5    12     1    ;     C     C
>>     6    13     1    ;     C     C
>>     6    26     1    ;     C    2H
>>     6    27     1    ;     C    1H
>>     7     9     1    ;     C     C
>>     7    28     1    ;     C    2H
>>     7    29     1    ;     C    1H
>>     8    10     1    ;     C     C
>>     8    14     1    ;     C     C
>>     9    13     1    ;     C     C
>>     9    16     1    ;     C     C
>>    10    15     1    ;     C     C
>>    11    30     1    ;     C    1H
>>    11    31     1    ;     C    2H
>>    11    32     1    ;     C    3H
>>    12    33     1    ;     C    1H
>>    12    34     1    ;     C    2H
>>    12    35     1    ;     C    3H
>>    *13    36     1    ;     C     H*
>>
>>    14    18     1    ;     C     C
>>    15    17     1    ;     C     C
>>    15    37     1    ;     C     H
>>    16    38     1    ;     C    1H
>>    16    39     1    ;     C    2H
>>    16    40     1    ;     C    3H
>>    17    18     1    ;     C     C
>>    17    19     1    ;     C     C
>>    18    41     1    ;     C     H
>>    19    20     1    ;     C     C
>>    19    42     1    ;     C    1H
>>    19    43     1    ;     C    2H
>>    20    21     1    ;     C     C
>>    20    44     1    ;     C    2H
>>    20    45     1    ;     C    1H
>>    21    22     1    ;     C     C
>>    21    47     1    ;     C    1H
>>    21    48     1    ;     C    2H
>>    22    23     1    ;     C     C
>>    22    49     1    ;     C    2H
>>    22    50     1    ;     C    1H
>>    23    51     1    ;     C    1H
>>    23    52     1    ;     C    2H
>>    23    53     1    ;     C    3H
>>
>> On Tue, Feb 28, 2017 at 3:57 AM, Justin Lemkul <jalemkul at vt.edu> wrote:
>>
>>
>>>
>>> On 2/27/17 12:49 AM, RAHUL SURESH wrote:
>>>
>>> I am explaining my issues regarding complex simulation.
>>>>
>>>> I have 200ns conformer(OPLS FF)
>>>>
>>>> I have generated my ligand gro and itp file.(Using topolgen1_1)
>>>>
>>>> I have edited my protein gro file and made it into complex file as per
>>>> the
>>>> tutorials.
>>>>
>>>> I have made changes to my topol.top file.
>>>>
>>>> Now in my command for em.mdp I am getting 19 errors stating "NO DEFAULT
>>>> BOND TYPE, ANGLE TYPE, ETC."
>>>>
>>>> Can you guide me with this?
>>>>
>>>>
>>>> This means the atom type assignment makes reference to bonded parameters
>>> that don't exist.  That means one of two things: (1) either the atom type
>>> assignment is incorrect, requiring manual correction (as the script warns
>>> you, as it makes a lot of assumptions and is therefore rather dumb
>>> sometimes) or (2) you simply have a molecule for which parameters do not
>>> exist, requiring manual parametrization.  For OPLS-AA, this requires QM
>>> calculations to get things like equilibrium geometries, vibrational
>>> frequencies, and dihedral potential energy scans.
>>>
>>>
>>> -Justin
>>>
>>> --
>>> ==================================================
>>>
>>> Justin A. Lemkul, Ph.D.
>>> Ruth L. Kirschstein NRSA Postdoctoral Fellow
>>>
>>> Department of Pharmaceutical Sciences
>>> School of Pharmacy
>>> Health Sciences Facility II, Room 629
>>> University of Maryland, Baltimore
>>> 20 Penn St.
>>> Baltimore, MD 21201
>>>
>>> jalemkul at outerbanks.umaryland.edu | (410) 706-7441
>>> http://mackerell.umaryland.edu/~jalemkul
>>>
>>> ==================================================
>>> --
>>> Gromacs Users mailing list
>>>
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>>>
>>>
>>
>>
>>
> --
> ==================================================
>
> Justin A. Lemkul, Ph.D.
> Ruth L. Kirschstein NRSA Postdoctoral Fellow
>
> Department of Pharmaceutical Sciences
> School of Pharmacy
> Health Sciences Facility II, Room 629
> University of Maryland, Baltimore
> 20 Penn St.
> Baltimore, MD 21201
>
> jalemkul at outerbanks.umaryland.edu | (410) 706-7441
> http://mackerell.umaryland.edu/~jalemkul
>
> ==================================================
> --
> Gromacs Users mailing list
>
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>



-- 
*Regards,*
*Rahul Suresh*
*Research Scholar*
*Bharathiar University*
*Coimbatore*


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